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Table 1 Total amount of RNAs in a single yeast cell grown in YPD medium

From: Absolute quantification of the budding yeast transcriptome by means of competitive PCR between genomic and complementary DNAs

Method

Culture #1

Culture #2

Culture #3

Average (SD)

Hot Phenol

1.32

1.34

1.29

1.32 (0.03)

NaOH/PCA

1.43

1.45

1.43

1.43 (0.01)

  1. Total amount of cellular RNAs (pg per cell) was determined for three independent preparations of S288C cells grown in YPD medium using a modified hot-phenol method [12] and NaOH/PCA method [13].
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BMC Genomics

ISSN: 1471-2164

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