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Table 2 Oligonucleotides used as primers and adaptors

From: Absolute quantification of the budding yeast transcriptome by means of competitive PCR between genomic and complementary DNAs

Name

Sequence

Adaptor-specific primer

M13RV

5' -CAGGAAACAGCTATGAC-3'

Adaptor

Forward oligonucleotide

A

5' -TGCACAATACTCACACAGGAAACAGCTATGACTGCGCTCACATCG-3'

B

5' -ACAATTCACACAGGAAACAGCTATGACTGCACTGCGCTCACATCG-3'

Reverse oligonucleotide

C

5' -(PO4)GATCCGATGTGAGCGCCA-3'

  1. The Y-shaped adaptor A/C or B/C was prepared by annealing equal molar amounts of the forward oligonucleotide A or B and the reverse oligonucleotide C, respectively. The sequence shared by the adaptor specific primer (ASP) and the adaptors is shown in bold. Complementary sequences between the forward and reverse oligonucleotides are underlined.
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BMC Genomics

ISSN: 1471-2164

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