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Table 1 Comparison of fold-change values from qRT-PCR and microarray for selected target genes.

From: Development and validation of a gene expression oligo microarray for the gilthead sea bream (Sparus aurata)

TARGET TRANSCRIPT

SAPD ID

Fold Changea

  

Real-time RT-PCR

Microarray Probe_1

Microarray Probe_2

Apolipoprotein E1

SAPD02358

0.00362

0.12263

0.1276

Flap endonuclease 1

SAPD04884

0.07811

0.16678

0.18717

Ovostatin

SAPD01680

0.00525

0.13731

0.14149

Myosin

SAPD10294

0.16533

0.25064

0.21589

Serotransferrin

SAPD01126

0.02439

0.11883

0.10408

Glutamate R7

SAPD19202

3.58394

4.30343

4.61982

Methionine aminopeptidase 2

SAPD26496

12.9384

6.88419

6.91868

L-lactate dehydrogenase

SAPD00597

3.09142

2.99927

3.00815

Serine racemase

SAPD19150

40.9397

18.3846

19.9929

Retinal cone arrestin-3

SAPD02277

2232.6

29.4717

44.2798

Phosphoglycerate kinase 1

SAPD03464

1.84119

1.26289

1.12021

Malate dehydrogenase 1

SAPD02236

 

1.05

0.99

  1. a Fold change is calculated as ratio of Stage 4 vs Stage 1, using mean signal intensity across five biological replicates of each stage. Values below 1.0 indicate down-regulation in Stage 4.
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BMC Genomics

ISSN: 1471-2164

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