Figure 2

a. A log10 scale scatter plot comparison of the transcript sequence tag counts as assessed by SAGE analysis between the HMD (y-axis) and the LMD (x-axis) libraries. Significant differentially expressed transcripts with a P-value = 0.05 with higher expression in HMD are indicated by pink triangles and those with higher expression in LMD are indicated by blue triangles. Points colored in grey are considered non-significant (P-value > 0.05). A few representative genes are indicated by their corresponding gene symbol. Pde9a, phosphodiesterase 9A; Nolz1, zinc-finger protein Nolz1; Pan3, Pan3 polyA specific ribonuclease subunit homolog; HSP90ab, heat shock protein 90 kDa alpha, classB1; Wsb2, WD repeat and SOCS box-containing 2. 2b. Relative mRNA expression was measured by real-time PCR using SYBR-Green as described in Materials and Methods. Comparison between HMD/high responders (n = 6) and LMD/low responders (n = 6) in whole lung tissue obtained from challenged C57/BL6 mice. The values for the HMD samples were arbitrarily set to 1 in order to display the fold difference. P-values were calculated according to the Mann-Whitney U Test: Nfact1 = 0.004; Jak2 = 0.077; Rcor3 = 0.002; Tmem151a = 0.179; Dnmt3A = 0.051; Dnmt3B = 0.041; Dnmt1 = 0.002. All data are presented as the mean ± standard error of the mean.