Figure 3

Transcriptional Status of Human GLTP Genes. A. Agarose gel electrophoresis patterns of PCR products (37 cycles) obtained with 'universal GLTP primers' from different cells. Odd numbered lanes = RT-PCR; Even numbered lanes = No-RT PCR controls (PCR with no reverse transcriptase); Lanes 1 & 2 = glioma cells; Lanes 3 & 4 = human skin fibroblasts; Lanes 5 & 6 = human HBL100 breast cancer cells; Lanes 7 & 8 = Gaucher cells; Lanes 9 & 10 = human T47D breast cancer cells; Lanes 11 & 12 = human IMR32 neuroblastoma cells; Lanes 13 & 14 = human HTB126 breast cancer cells; Lanes 15 & 16 = human Caov3 ovarian cancer cells. β-actin transcription is shown in subtending panel at 30 PCR cycles. B. Agarose gel electrophoresis patterns obtained after Bsp HI restriction digestion of pGEM-T clones containing GLTP ORFs originating from 5-exon/4-intron GLTP (Lane 1) or from intronless GLTP (Lane 2). Std = molecular weight standards.