Figure 3From: The Sterolgene v0 cDNA microarray: a systemic approach to studies of cholesterol homeostasis and drug metabolismNormalization of Sterolgene v0 microarray data. MA plots of Sterolgene v0 microarray data illustrating its normalization. Genes are marked with crosses (+), Ambion ArrayControl spikes with circles (○) and diamonds (◇), and Firefly luciferase mRNAs with squares (□). A: data before normalization together with LOWESS curve through normalization spike in RNAs. C: the same data as shown in A, but with normalization controls adjusted according to their RNA concentrations (see text for details). B and D: data after normalization according to LOWESS curves shown in A and C, respectively. Adjusted are 4 types of Ambion spikes marked with green, orange and red circles/diamonds.Back to article page