Figure 6

Changes in cholesterol homeostasis and drug metabolism caused by different factors in mouse liver. With Sterolgene array we successfully detected all changes in cholesterol homeostasis and drug metabolism caused by high-cholesterol diet, fasting, TNF-α, and phenobarbital (PB) treatment (solid arrows). With Agilent array (G4104A) we detected none of the changes caused by the inflammatory cytokine TNF-α and fasting (crosses). With Affymetrix MOE430A GeneChip we detected only down-regulation of the cholesterol biosynthesis by the high-cholesterol diet and induction of the Cyp2b family by the phenobarbital treatment (dashed arrows), but not the up-regulation of Cyp3a11 by high-cholesterol diet and up-regulation of Cyp3a family and Alas1 by phenobarbital treatment. In all experiments, two tailed Student's t-test was used for classification of differentially expressed genes.