Table 2 Differentially expressed genes in the mouse liver after TNF-α treatment and fasting (Sterolgene v0 microarray). Differentially expressed genes in the mouse liver after TNF-α treatment/fasting as detected by the Sterolgene array (α = 0.05). Genes in bold – RT-PCR analysis is consistent with microarray data.
Gene name and function | Gene symbol | Log2 ratio | GeneBank Acc. No. |
|---|---|---|---|
Cholesterol biosynthesis | |||
Lanosterol 14α-demethylase | Cyp51a1 | 1.35 | BC031813 |
Sterol-C4-methyl oxidase-like | Sc4mol | 1.63 | NM_025436 |
Bile acid biosynthesis | |||
Sterol 12α-hydroxylase | Cyp8b1 | -1.82 | NM_010012 |
Cytochrome P450 superfamily | |||
Cytochrome P450, 2f2 | Cyp2f2 | -1.72 | NM_007817 |
Cytochrome P450, 2j5 | Cyp2j5 | -0.68 | NM_010007 |
Cytochrome P450, 3a25 | Cyp3a25 | -1.56 | BC028855 |
Cholesterol side chain cleavage enzyme | Cyp11a1 | -0.30 | NM_019779 |
Nuclear receptor superfamily | |||
Testis receptor | Nr2c1 | -1.31 | NM_011629 |
Glucocorticoid receptor | Nr3c1 | -0.97 | NM_008173 |
Androgen receptor | Nr3c4 | -0.71 | M37890 |
Other | |||
Glyceraldehyde-3-phosphate dehydrogenase | Gapd | 0.53 | NM_008084 |