Effect of thyroid hormone concentration on the transcriptional response underlying induced metamorphosis in the Mexican axolotl (Ambystoma)

Table 3 Primer sequences used for Q-RT-PCR

Gene	Symbol	Probe-set ID/Direction	Sequence
Desmin	DES	SRV_01373_at_5.1	GGGCCAATTATGAAGCAA
		SRV_01373_at_3.1	CTTCGACGACTCGCTTTG
Desmocollin 1	DSC1	SRV_04693_a_at_5.1	GAGTCATTGCTCTTCCTTGG
		SRV_04693_a_at_3.1	TTTTGATGTGCTGCTCCA
Matrix metallopeptidase 13	MMP13	SRV_11423_at_5.1	TGGAAGAAGACTGCGTTGA
		SRV_11423_at_3.1	ACACTTTGAAGGCCCTTTG
Solute carrier family 31 member 1	SLC31A1	SRV_01215_a_at_5.1	TGTGATCAAACCCCAGGA
		SRV_01215_a_at_3.1	CGCGCAATCTTAAGGAAC
N/A	N/A	SRV_10216_s_at_5.1	ACATCACTGTCCCCGAAGT
		SRV_10216_s_at_3.1	GGTCGGGGTCTGTTCAAT

N/A refers to a gene that does not have an established orthology with human. Probe-set ID represents the unique identifier for each probe-set on the custom Ambystoma GeneChip. Forward and reverse primers are denoted by the extensions 5.1 and 3.1 respectively.

ISSN: 1471-2164