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Table 4 Reproducibility of QSS on mixtures of plasmid DNA

From: Quantitative Single-letter Sequencing: a method for simultaneously monitoring numerous known allelic variants in single DNA samples

 

Percentage of each VIT

Total (%)

Replicates of Mix N°9

VIT1

VIT2

VIT3

VIT4

VIT5

VIT6

 

1

16.92

16.78

16.55

16.72

16.59

16.43

100

2

17.45

16.99

17.14

16.12

16.19

16.12

100

3

17.17

16.58

16.81

16.74

16.79

15.92

100

4

17.06

16.47

16.32

16.68

17.33

16.14

100

5

17.19

16.36

17.03

16.60

17.21

15.62

100

Mean

17,16

16,63

16,77

16,57

16,82

16,05

100

SD

0.195

0.251

0.336

0.261

0.465

0.301

 
  1. Reproducibility of QSS as evaluated by the standard deviation (SD) on 5 replications of the analysis of Mix n°9. Proportional correction of the frequency of all markers was applied to give a total of 100% in each mix.
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BMC Genomics

ISSN: 1471-2164

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