Figure 6

Quantitative real-time PCR validation of the differentially expressed genes. The relative expression of a candidate gene was normalized against RpL3. For the up-regulated DEGs in the domestic silkworms, the fold-change of each gene was calculated by dividing the relative expression level in the W_AKSQ. For the up-regulated DEGs in the wild silkworms, the fold-change of each gene was calculated by dividing the relative expression level in the D_CH. The data are the average ± standard error of three independent replicated qPCR experiments. An absolute value of fold-change ≥ 2 and one-way analysis of variance analysis (P-value < 0.005) were used to estimate the significance of gene expression changes. Significant differential expressions of genes between any two silkworms were marked by a star.