Figure 5

Quantitative RT-PCR validations of microarray data in Oded and Hermoza fruits. A) The comparison of the microarray and qRT-PCR assay data, based on log2 data in Od and Hz. A total 10 differentially expressed genes were chosen, representing 60 comparisons where one gene covers 3 different time points (H, CS1 and CS2). Line shown represents the orthogonal fit to the data and correlation (R) is shown. B) Example of gene expression profiles across H, CS1 and CS2 samples in Od and Hz determined by qRT-PCR and microarray on four peach genes previously associated to CI tolerance (IAA27, Thaumatin-1-like, ACS and ACO). In the graphs there are represented Od and Hz values at harvest (bars) and the average gene expression pattern relative to harvest values in both platforms, microarray and qRT-PCR.