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Table 3 Genes discussed in the text correlated with tolerance

From: Pre-symptomatic transcriptome changes during cold storage of chilling sensitive and resistant peach cultivars to elucidate chilling injury mechanisms

Function Specific process

Chillpeach ID

Unigene annotation

Arab AGI

Arab gene symbol

Hormone signaling

Sugar signaling/ partioning

Hormone and secondary metabolite biosynthesis regulation

Cell wall and cytoesqueleton related

Cell polarity

k-means 5. Correlated with tolerance at harvest and during cold storage

Aminoacid metabolism

 

Cyanide detoxification

PPN075E10

Beta-cyanoalanine synthase 1

AT3G61440

CYSC1

     
 

Methionine metabolism

PPN034A06

1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 4

AT5G43850

ARD4

  

Yang Cycle

associated to VN tissue

 
  

PPN034C12

1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 3

AT4G14710

ARD2

  

Yang Cycle

associated to VN tissue

 
  

PPN072E05

Cystathionine gamma synthase

AT3G01120

MTO1/CGS1

     

Antioxidant system

 

GLUTHATHIONE-GLUTAREDOXIN AND THIOREDOXIN REDOX HOMEOSTASIS

PPN039H11

Glutathione S-transferase

AT5G17220

TT19/GSTF12

  

PA monomer transporter

  

Cytoskeleton organization and biogenesis

 

Actin microfilament-actin depolimerization

PPN047E05

Actin depolymerizing factor 2

AT5G59880

ADF3

     
 

Microtubule-Microtubule binding and stabilization

PPN073D05

Microtubule-associated proteins

AT5G55230

MAP65-1

     
 

Microtubule-microtubule organization and formation

PPN075E12

Tubulin folding cofactor B

AT3G10220

EMB2804/ TFC

     

RNA transcription regulation

 

AP2/ EREBP family

PPN054F05

AP2-related transcription factor

AT5G47220

ERF2

ET signaling

  

VN cell division

 
 

AUX/IAA family

PPN014H03

Auxin-induced protein AUX28

AT1G04250

AXR3/IAA17

AUX and ABA nuclear signaling; negative regulator

    
  

PPN057F01

AUX/IAA protein

AT4G29080

PAP2/IAA27

AUX nuclear signaling; negative regulator

    
 

LIM-family

PPN009B01

Pollen-specific protein SF3, putative

AT1G10200

WLIM1

     
  

PPN069C01

Transcription factor lim1

AT1G10200

WLIM1

   

Actin stabilizing protein

 
 

MADS-box family

PP1006G03

MADS-box transcription factor

AT5G60910

FUL/ AGL8

  

Positive regulatior of caroterne and anthocyanin biosynthesis,

Negative regulation of lignin

 
  

PPN042H02

MADS4

AT4G18960

AG

ET up-regulation

 

Postisitive carotene biosynthesis regulation;

Negative regulation of lignin biosynthesis

 

RNA translation and protein assembly

 

Regulation of protein biosynthesis

PPN006H04

Translationally-controlled tumor protein homolog

AT3G16640

TCTP

AUX cytoplasmic signaling

Sugar signaling

 

CW biosynthesis regulation

 

Secondary metabolism

 

Anthocyanin metabolism

PpLDOX (PpLDOX)

Leucoanthocyanidin dioxygenase

 

PpLDOX

  

Flavonoid/PA biosynthesis

  
  

PPN055C03

Anthocyanidin reductase

AT1G61720

BAN

  

PA biosynthess

  
 

Aspartate biosyntheis

PPN046D06

1-aminocyclopropane-1-carboxylate synthase

AT1G62960

ACS10

     
 

Carotenoid metabolism

PPN006A10

Phytoene synthase

AT5G17230

PSY

     
  

PPN067A01

Capsanthin/ capsorubin synthase

AT3G10230

LYC

     
 

Cyanide detoxification

PP1000E01

Cyanate hydratase

AT3G23490

CYN

     
  

PPN066B01

Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein

AT5G12040

      
 

Flavonoid metabolism

PPN050G05

Dihydroflavonol 4-reductase-like

AT5G58490

   

Flavonoid/PA biosynthesis

  
  

PPN052H09

Chalcone synthase 2

AT5G13930

CHS/TT4

Negative regulation of AUX transport

    

Signal transduction pathway

 

Cytoplasmic TOR signaling

PPN076G10

Protein lethal with sec thirteen 8-2

AT3G18140

LST8-1

AUX cytoplasmic signaling

Sugar signaling

 

CW biosynthesis regulation

 
 

ET signaling/ ET signal transduction

PPN011G11

GTP-binding protein

AT3G46060

ARA3/RAB8A

ET signaling

    

Trafficking machinery and membrane dynamics

 

CME;EE;internalization and intracellular trafficking of PM proteins

PPN011F03

Clathrin_L-chain

AT2G40060

CLC2

Regulates cellular AUX levels by controlling the abundance and distribution of PIN proteins at the PM

   

Cell polarity regulation

 

CME;internalization and intracellular trafficking of PM proteins

PPN017G03

Calcium-binding EF-hand

AT3G01780

TPLATE

Regulates cellular AUX levels by controlling the abundance and distribution of PIN proteins at the PM

  

Regulation of cellulose synthesis by controlling the abundance of active CESA complexes at the PM

Cell polarity regulation

 

Endosomal sorting complex

PPN060A04

Putative endosomal Vps protein complex subunit

AT5G22950

VPS24.1

Required for internalize PIN1, PIN2, and AUX1 to the MVB/ LE for vacuolar degradation

    
 

Golgy to ER/ COPI vesicles

PPN044E10

ARF-like small GTPase 1

AT2G47170

ARF1A1C/BEX1

Essential for recycling of PIN transporters to the PM and for vacuolar targeting

   

Cell polarity

 

Retromer complex;LE to vacuole

PPN007G03

Sorting nexin-like protein

AT5G06140

SNX1

Regulates both the recycling VSR from the TGN/ EE to the ER and the balance between vacuolar degradation and recycling of PIN proteins

    
  

PPN023B01

Ras-related protein Rab7

AT3G18820

RABG3F/RAB7B

     

k-means 8. Associated with high tolerance to chilling injury

Aminoacid metabolism

 

AUX biosynthesis

PPN058D11

Anthranilate synthase beta subunit

AT1G25220

ASB1

AUX biosynthesis

    

RNA transcription regulation

 

AUX/ IAA family

PP1009D02

IAA16 protein

AT1G04250

AXR3/IAA17

AUX and ABA nuclear signaling; negative regulator

    
  

PPN060G07

AUX/IAA protein

AT1G04240

IAA3/SHY2

AUX nuclear signaling; negative regulator

    
 

HD-ZIP family

PPN074H05

HB2 homeodomain protein

AT4G35550

HB-4/WOX13

AUX regulated

  

SCW biosynthesis; negative regulator lignin biosynthesis

 
 

MYB-family

PPN067A04

MYB-like DNA-binding domain protein

AT4G38620

MYB4

   

SCW biosynthesis; negative regulator lignin biosynthesis

 

Signal transduction pathway

 

ET signaling/ ET receptor

PPN054G06

Ethylene receptor

AT3G04580

EIN4

ET signaling

    

Transport

 

Cooper transport

PPN035H02

Copper-transporting ATPase RAN1

AT5G44790

RAN1

ET signaling; delivers cooper ion into the ET receptors; is required for both ET binding and the receptor functionality

    
  1. Abbreviations: AUX:auxin; ET; ethylene; ABA: Abcisic acid; PM:plasma membrane; CW: cell wall; SCW: secondary cell wall; ER: endoplasmic reticulum; MVB/LE: microvesicular body/late endosome; TGN/EE:trans-golgy network/early endosome; VSR: vacuolar sorting receptors VN:vascular networks; PA: proanthocyanines; PIN; PIN formed auxin efflux carrier; RG: rhamnogalacturonan; XyG: xyloglucan.
  2. References supporting information in Table 3 are provided in Additional file 8: Table S7.