Table 1 Summary of sequence counts from small RNA libraries. Samples were obtained from different developmental stages and four tissues (brain, gonad, liver, and pituitary) of Atlantic cod
From: Temperature during early development has long-term effects on microRNA expression in Atlantic cod
Description | Development count | Tissues count |
|---|---|---|
Raw SOLiD reads | 303 165 498 | 268 844 467 |
Adapter only sequences | 676 039 | 605 210 |
Reads without 3’ adapter sequence | 42 928 670 | 67 064 659 |
Low quality sequences | 24 440 083 | 27 931 725 |
Size ≤ 15 nt | 10 286 414 | 4 750 802 |
Artifact sequences | 83 923 | 60 426 |
High quality, filtered sequences | 224 750 369 | 168 431 645 |
Unique sequences | 23 084 734 | 24 341 799 |
miRBase v20 alignment | ||
No mismatches | 49 845 256 | 67 205 304 |
1 mismatches | 9 025 096 | 11 872 566 |
Types of conserved mature miRNAs | 281 | 281 |