The complete methylome of Helicobacter pylori UM032

BMC Genomics

Table 1 Methylated motifs detected for H. pylori UM032

Type of RM system	Motifs ^a	Type of modification	No. detected ^d	No. in genome	% detected	Locus tag	Nomenclature
I	GANNNNNNNTAYG	m6A	653	653	100.00	K747_03505	M.HpyUM032XII
IIP	TCGA	m6A	526	526	100.00	K747_09985	M.HpyUM032XVII
IIP	CA TG	m6A	14370	14370	100.00	K747_04980	M.HpyUM032I
IIP	ACNGT	m4C	1005	1104	91.03	K747_10995	M.HpyUM032II
IIP	GA TC	m6A	10172	10172	100.00	K747_09245	M.HpyUM032III
IIP	GANTC	m6A	5388	5388	100.00	K747_12490	M.HpyUM032IV
IIP	CCGG	m4C	3396	3416	99.41	K747_10000	M.HpyUM032IX
IIP	TCNGA	m6A	2530	2532	99.92	K747_05140	M.HpyUM032V
IIP	ATTAAT	m6A	857	874	98.05	K747_10980	M.HpyUM032VII
IIP	TGCA	m6A	11260	11270	99.91	K747_12120	M.HpyUM032VIII
IIS	GAGG	m6A	4578	4579	99.98	K747_08850	M2.HpyUM032VI
IIS	CCATC	m6A	2255	2255	100.00	K747_03690	M1.HpyUM032X
IIP	GC GC^e	m5C	774	2396	32.30	K747_05430	M.HpyUM032XV
IIG	CYANNNNNNNTRG^b	m6A	2319	2320	99.96	K747_03825	HpyUM032XIII
IIG	GAAAG^b	m6A	2514	4955	50.74	K747_03595	HpyUM032XIV
IIP	GTNNAC	m6A	528	528	100.00	K747_06370	M.HpyUM032XI
IIP	GT AC	m6A	174	174	100.00	K747_06575	M.HpyUM032XVIII
I	CCANNNNNNTC^b,c	m6A	-	-	-	K747_10905	M.HpyUM032XVI

^aThe methylated base within the motif is in bold while the methylated base in the complementary strand is italic.
^bNovel recognition sequences.
^cActivity identified only after cloning. No methylation activity was observed in H. pylori UM032.
^dThe total number includes motifs occurring on the “+” and “–” strands.
^eLow percentage detected, due to m5C modification.

ISSN: 1471-2164