Deep developmental transcriptome sequencing uncovers numerous new genes and enhances gene annotation in the sponge Amphimedon queenslandica

Table 1 Transcriptome sequencing statistics

	Precompetent larvae	Competent larvae	Juvenile	Adult	Adult deep
Reads	42,273,865	43,699,007	41,677,487	43,098,690	125,880,671
Quality trimmed reads	39,234,338	40,546,526	38,587,047	39,976,627	116,384,700
Assembled transcripts	107,725	122,306	101,432	112,187	230,181
Average transcript length	816	738	706	716	1,278
Longest transcript	14,660	12,052	9,354	16,961	29,513
Mapped Transcripts	63,681	64,826	61,149	65,377	76,045
% Mapped/Assembled	59.11%	53.00%	60.29%	58.28%	33.04%
Average mapped transcript	738	729	673	686	918
Longest mapped transcript	14,393	12,052	24,959	16,954	29,166
Total transcripts mapped to genome	63,681	64,826	61,149	65,377	76,045
Total coverage (% genome)	28.32%	27.92%	29.24%	30.31%	21.62%
Used for PASA	Yes	Yes	Yes	Yes	No

ISSN: 1471-2164