Characterization of a second secologanin synthase isoform producing both secologanin and secoxyloganin allows enhanced de novo assembly of a Catharanthus roseus transcriptome

Table 1 Transcript annotation and analysis of full-length transcript reconstruction

Assembly	ccOrcae	mpgrCra	NIPGR	PMS454	PMSIllu	CD97	CD97 best clusters^c	CDF97
Number of transcripts	31,450	86,726	59,220	26,804	155,305	543,979	31,055	58,338
Total annotated transcripts^a	16,727	55,073	24,142	15,868	22,716	249,413	25,692	49,128
% Annotated transcripts^a	53.19	63.50	40.77	59.20	14.63	45.85	82.73	84.21
	Cumulative number of proteins^b
% Length coverage
100	4,539	4,115	3,540	1,546	3,168	7,155	5,006	5,734
90	5,686	5,547	5,124	2,127	4,125	9,283	6,198	7,110
80	6,370	6,626	6,377	2,624	4,783	10,903	6,913	7,926
70	6,855	7,641	7,469	3,193	5,381	12,410	7,466	8,572
60	7,298	8,703	8,372	3,827	5,940	14,048	7,958	9,135
50	7,724	9,670	9,119	4,575	6,574	16,085	8,350	9,640
40	8,102	10,528	9,686	5,457	7,177	18,610	8,671	10,033
30	8,479	11,282	10,141	6,497	7,793	21,831	8,908	10,489
20	8,767	11,744	10,393	7,367	8,303	24,619	9,021	10,520
10	8,816	11,830	10,438	7,588	8,430	25,229	9,044	11,137

^a,Blastx analysis vs UniprotKB/Swiss-Prot. A transcript was considered to be annotated if it matches a protein in the database at a e-value threshold of 1e-20
^b,Reports the cumulative number of proteins in UniprotKB/Swiss-Prot matched by at least one transcript in the corresponding assembly at a given % coverage
^c,Clusters in CD97 having contigs from at least ten different single assemblies

ISSN: 1471-2164