Fig. 2

Hierarchical clustering of statistically differentially regulated B. dorsalis miRNAs. The clustering allowed for the classification of groups of miRNAs with increased expression during the developmental progression from embryo to larva, embryo to pupa and embryo to female reproductive tract (group 1). A group of potential larva-specific miRNAs (group 2), and a group of miRNA with very low expression in pupa (group 3). The values used for clustering were in the form of log2 ratios to give equal weight to up-regulated miRNAs and down-regulated miRNAs (e.g. MalePupa/Embryo means the log2 of the ratio of MalePupa counts per million over Embryo counts per million). Enclosed in rectangles are genes with orthology to two of the known D. melanogaster miRNA clusters