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Table 1 Sequenced libraries for each stage of development with total number of generated bases, total of reads per library, percent G + C, percent of reads with quality above Q20 or above Q30

From: De novo transcriptome assembly and analysis to identify potential gene targets for RNAi-mediated control of the tomato leafminer (Tuta absoluta)

Libraries

Total bases

(G + C)%

Q20 (%)

Q30 (%)

Total reads

Filtered reads

Assembled

Eggs

5,626,900,284

43.53

94.4

87.4

55,711,884

52,617,684

19,261,127

1st Stage

3,718,827,070

41.23

94.8

88.0

36,820,070

28,657,483

12,957,321

2nd Stage

3,497,118,738

43.91

94.2

87.0

34,624,938

32,621,354

12,777,709

3rd Stage

4,469,182,734

45.21

93.9

86.4

44,249,334

41,546,244

13,442,005

4th Stage

3,759,123,848

45.56

94.1

86.6

37,219,048

35,045,786

9,689,430

Adults

3,668,050,734

44.01

94.0

86.5

36,317,334

34,105,903

14,378,063

Total

24,739,203,408

   

244,942,608

 

82,505,655

Mean

 

44.0

94.2

87.0