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Table 2 Validation of ISMapper using real Illumina reads for which finished genomes were also available

From: ISMapper: identifying transposase insertion sites in bacterial genomes from short read sequence data

Isolate

Genome accession

FastQ accession

IS

Found

Orientation

Streptococcus suis P1/7

AM946016

ERR225612

ISSu3

4/4

4/4

  

ISSu4b (2)

2/2

2/2

Staphylococcus aureus TW20

NC_017331

ERR043367

ISSep3

3/3

3/3

  

IS256

8/8

8/8

  

IS431meca

3/4

3/4

  

IS1181

2/2

2/2

Klebsiella pneumoniae NJST258_1

CP006923

SRR1166975

ISKpn1

5/5

5/5

  

IS5B

8/8

8/8

  

IS903Bb (1)

2/2

2/2

  

IS1294

3/3

3/3

  

ISKpn18

2/2

2/2

  

ISKpn26

7/7

7/7

S. Typhi CT18

NC_003198

ERR343331

IS200

26/26

26/26

  

IS1 a

2/3

2/3

S. Typhi Ty2

AE014613

ERR343332

IS200

26/26

26/26

  1. aindicates ISMapper was unable to resolve some positions due to repeat regions
  2. bindicates ISMapper incorrectly identified an insertion site, the number of these sites are indicated in brackets