Fig. 5From: Early transcriptional events linked to induction of diapause revealed by RNAseq in larvae of drosophilid fly, Chymomyza costata Schematic depiction of experimental design. Larvae of Chymomyza costata were sampled on day 3 of their 3rd instar, which corresponds to the stage of maximum sensitivity to photoperiodic signal [54]. Larvae were exposed to different photoperiodic conditions: constant Long Days (LD) since embryonic stage promoting direct development to pupa (pupariation on day 10 in average); constant Short Days (SD) since embryonic stage inducing larval diapause (initiated between days 10 and 40); and Transfer (T) from LD to SD switching the direct development to diapause. The samples were taken at two Zeitgeber times in each photoperiodic conditions (i.e. LD1 and LD13; SD1 and SD17; T13 and T17). See text for more explanationsBack to article page