Table 1 Summary of 25 genes producing RNAi phenotypes
Gene | E–value | KOG group | Functional category | Phenotype |
|---|---|---|---|---|
Smed–slbp | 1.00E–10 | Histone mRNA stem–loop binding protein | [A] RNA processing and modification | Loss of stem cells |
Smed–sart3 | 4.00E–102 | RNA–binding protein SART3 | [A] RNA processing and modification | Loss of stem cells |
Smed–smarcc–1 | 2.00E–08 | Chromatin remodeling factor subunit and related transcription factors | [B] Chromatin structure and dynamics | Loss of stem cells |
Smed–cycD | 2.00E–10 | G1/S–specific cyclin D | [D] Cell cycle control, cell division, chromosome partitioning | Loss of stem cells |
Smed–espl1 | 3.00E–32 | Regulator of spindle pole body duplication | [D] Cell cycle control, cell division, chromosome partitioning | Loss of stem cells |
Smed–rrm2b–1 | 6.00E–28 | Ribonucleotide reductase, beta subunit | [F] Nucleotide transport and metabolism | Loss of stem cells |
Smed–rrm2b–2 | 3.00E–143 | Ribonucleotide reductase, beta subunit | [F] Nucleotide transport and metabolism | Loss of stem cells* |
Smed–pdss2/dlp1 | 1.00E–28 | Geranylgeranyl pyrophosphate synthase/ Polyprenyl synthetase | [H] Coenzyme transport and metabolism | Loss of stem cells* |
Smed–dkc1 | 5.00E–149 | Pseudouridine synthase | [J] Translation, ribosomal structure and biogenesis | Loss of stem cells |
Smed–emg1/nep1 | 1.00E–26 | Protein required for 18S rRNA maturation and 40S ribosome biogenesis | [J] Translation, ribosomal structure and biogenesis | Loss of stem cells* |
Smed–lig1 | 9.00E–125 | ATP–dependent DNA ligase I | [L] Replication, recombination and repair | Loss of stem cells |
Smed–prim2 | 1.00E–99 | Eukaryotic–type DNA primase, large subunit | [L] Replication, recombination and repair | Loss of stem cells |
Smed–mcm7 | 0 | DNA replication licensing factor, MCM7 component | [L] Replication, recombination and repair | Loss of stem cells* |
PL08006B2E08 | –– | No significant homology | none | Loss of stem cells |
Smed–rbap46/48–2 | 3.00E–79 | Nucleosome remodeling factor, subunit CAF1/NURF55/MSI1 | [B] Chromatin structure and dynamics | Six feedings – reduced/delayed regeneration, reduced mitosis Three feedings – elongated photoreceptor pigment |
Smed–mcm2 | 2.00E–148 | DNA replication licensing factor, MCM2 component | [L] Replication, recombination and repair | Reduced/delayed regeneration, asymmetric photoreceptors, reduced mitosis |
Smed–ptbp1 | 2.00E–54 | Polypyrimidine tract–binding protein | [A] RNA processing and modification | Reduced/delayed regeneration, forked tail, inching movement, lysis |
Smed–fen–1 | 1.00E–149 | 5′–3′ exonuclease | [L] Replication, recombination and repair | Reduced/delayed regeneration |
Smed–morf4l1/mrg–1 | 5E–26 | Dosage compensation regulatory complex/ histone acetyltransferase complex, subunit MSL–3/MRG15/EAF3 | [BK] Chromatin structure and dynamics, transcription | Reduced/delayed regeneration, lysis |
Smed–ddc | 2.00E–57 | Aromatic–L–amino–acid/L–histidine decarboxylase | [E] Amino acid transport and metabolism | Faint photoreceptor pigment |
Smed–tph | 0 | Aromatic amino acid hydroxylase | [E] Amino acid transport and metabolism | No photoreceptor pigment, elongated, inching movement |
Smed–gas8 | 9.00E–87 | No significant homology | None | Edema |
Smed–pgbd4 | 1.00E–05 | No significant homology | None | Lesion at posterior of pharynx, dorsal hump, bulged sides, loss of pharynx, impaired photoreceptor development |
Smed–b9d2 | 9.00E–75 | Uncharacterized conserved protein | [S] Function unknown | Inching movement |
Smed–ima–1 | 1.00E–104 | Karyopherin (importin) alpha | [U] Intracellular trafficking, secretion, and vesicular transport | Collapse toward midline, cyclops/asymmetric photoreceptors, reduced mitosis |