Construction of a high-density mutant library in soybean and development of a mutant retrieval method using amplicon sequencing

Table 2 Mutations in 12 lines detected by using whole-genome re-sequencing analysis

Line name	Depth of coverage	Genome coverage (%)	Number of base changes	Type of base change												Amino acid substitutions	Misssense mutations	Nonsense mutations	Distance between base changes (kb)**
Line name	Depth of coverage	Genome coverage (%)	Number of base changes	G > A	C > T	T > A	A > T	T > C	A > G	G > T	C > A	A > C	T > G	C > G	G > C	Amino acid substitutions	Misssense mutations	Nonsense mutations	Distance between base changes (kb)**
EnT-0263	16.8	94.2	9934	3776	3481	635	718	266	300	202	206	135	117	60	38	573	543	30	95.7
EnT-0394	14.3	94.0	15579	5494	6493	1023	985	298	266	262	245	170	194	85	64	950	899	51	61.0
EnT-0442	17.0	94.1	8970	3612	3179	541	541	231	229	146	159	129	107	56	40	564	535	29	106.0
EnT-0541	23.1	93.9	15627	5666	5638	1120	966	459	452	338	334	237	220	103	94	823	785	38	60.8
EnT-0790	16.9	93.9	9366	3391	3366	661	609	262	252	171	212	155	160	65	62	514	485	29	101.5
EnT-0964	19.3	94.3	21861	8099	7688	1980	1972	397	373	374	324	265	263	61	65	1303	1246	57	43.5
EnT-1045	21.5	93.9	9172	2428	2926	1217	996	349	272	205	235	195	212	84	53	442	426	16	103.6
EnT-1079	18.0	94.0	14074	5376	5167	1057	919	292	297	271	247	183	150	69	46	902	865	37	67.5
EnT-1197	15.2	94.0	13782	4516	4877	1383	1234	353	295	269	289	194	227	79	66	796	756	40	69.0
EnT-1610	25.0	93.8	9481	2536	2874	1220	1048	388	338	250	226	220	227	91	63	439	425	14	100.3
EnT-1619	19.4	93.8	13274	5187	4122	1077	1269	273	291	318	250	191	170	66	60	729	686	43	71.6
EnT-1634	20.5	93.6	12434	3306	3282	1997	2138	331	362	228	220	233	224	63	50	684	646	38	76.5
Average	18.9	94.0	12796	4449	4424	1159	1116	325	311	253	246	192	189	74	58	727	691	35	74.3
Percentage of each type of base change*				34.8 %	34.6 %	9.1 %	8.7 %	2.5 %	2.4 %	2.0 %	1.9 %	1.5 %	1.5 %	0.6 %	0.5 %

*The percentage of each type of base change was calculated from a total each type of base change in 12 mutants divided by all base changes
**The distance between base changes was calculated from a total number of base changes per plant and the size of chromosome-scale assembly of the soybean genome (950,068,807 bp)

ISSN: 1471-2164