Tissue-specific transcriptomics and proteomics of a filarial nematode and its Wolbachia endosymbiont

BMC Genomics

Table 2 Wolbachia Transcriptome and Proteome Coverage in D. immitis Tissues

Sample	wDi mapped reads	wDi mapped bases (millions)	Average coverage (FPKM)/transcript	% of all predicted wDi transcripts detected^a	Number of wDi proteins detected
FBW	304,004	21.9	229.5	90	134	161
	304,004	21.9			92
	443,364	31.9			92
	443,364	31.9			59
FU	15,109	1.1	922.0	69	33
FU	82,897	6.0	922.0	69	33
FH	308,412	22.2	216.3	86	34
FH	202,058	14.5	216.3	86	34
FI	1152	0.08	1627.3	7.1	0
MBW	440,851	22.0	621.5	97	-
MBW	337,307	16.9	621.5	97	-
MI	1111	0.05	1536.2	10	-
MT	264	0.01	1367.9	8.3	-
MS	-	-	-	-	0
MH	-	-	-	-	62

^a Based on number of transcripts expressed (FPKM >0) per life cycle stage. Values from each biological replicate (tissue samples from different worms) analyzed by each method are presented. Values from each biological replicate (tissue samples from different worms) analyzed by each method are presented. Technical replicates were pooled prior to further analysis. Total number of unique proteins identified are presented for each tissue (bracketed for FBW biological replicates)

ISSN: 1471-2164