Fig. 3

Kinetics of SOCP DNA replication. S. pneumoniae R6 WT (a), R6-SOCP^R (b) and S. pneumoniae R6 transformed with R6-SOCP^R-derived alleles for genes spr0058 (c), spr0897 (d) or spr1443 (e) were infected with the lytic phage SOCP at a MOI of 0.1. Total DNA was extracted at baseline and at 15, 30, 45, 60 and 90 min after infection. Total DNA was digested with SspI before being hybridized with an [α-³²P]dCTP-labeled probe covering the holin gene of SOCP (top blots). DNA loading was controlled by hybridizing the blots with an [α-³²P]dCTP-labeled probe covering the spr1443 gene from S. pneumoniae R6 (bottom blots). Holin/spr1443 signal ratios are indicated in italics below the blots. Hybridizations were done in triplicates and representative blots are shown. f Plot of holin/spr1443 signal ratios for S. pneumoniae R6 WT (black), R6-SOCP^R (dark grey), R6^spr0058 (dashed dark grey), R6^spr0897 (dashed black), and R6^spr1443 (dashed light grey). For each strain, the ratios at each time point (except for R6^spr1443 at 90 min) were significantly different (p < 0.01 by two-way ANOVA) than those of R6 WT