Synthetic community | Experimental design | Nm cycles | Template concentration (ng) | Sequencing platform |
---|---|---|---|---|
EM/UM | Fusion primer | 25 | 1,5,10 | MS |
EM | Fusion primer | 25 | 2,5 | IT, 454 |
EM | Universal tailed tag | 5+15 | 1,10 | MS |
EM | Universal tailed tag | 8+15 | 1,10 | MS |
EM/UM | Universal tailed tag | 10+15 | 2,5 | MS |
EM/UM | Adapter ligation | 25 | 500–750 | PB |