Fig. 4From: Identification of Nicotiana benthamiana microRNAs and their targets using high throughput sequencing and degradome analysisExpression analysis of selected conserved and other known miRNAs in different N. benthamiana tissues. Total RNA was extracted from different tissues including, seedling (Se), root (R), leaf (L), stem (St), flower (F) from N. benthamiana plants used in our laboratory and from plants from Boyce Thompson Institute (leafBTI - LBTI, seedlingBTI - SeBTI). The RNA was separated on PAGE and transferred to nylon membranes for Northern blot analysis of the miRNAs. Oligonucleotide probes were used to detect specific miRNAs, and an U6-specific probe was used to detect U6 RNA as a loading control for each membraneBack to article page