Table 1 High-throughput sequencing statistics of N. benthamiana sRNAs

Seedling 1

Raw reads

4 353 615

Stem 1

Raw reads

2 673 839

Adaptor removed

3 192 563

1 106 502

Adaptor removed

2 468 386

858 314

Filter by sequence properties

3 187 558

1 102 305

Filter by sequence properties

2 464 474

854 744

t/rRNA removed

3 034 009

2 791 655

1 085 271

942 175

t/rRNA removed

2 404 489

2 205 954

846 701

746 712

Match known miRNAs

262 998

259 394

1 531

792

Match known miRNAs

121 361

119 819

1 376

860

Seedling 2

Raw reads

13 565 620

Stem 2

Raw reads

13 390 095

Adaptor removed

9 931 841

1 048 202

Adaptor removed

12 682 160

3 280 430

Filter by sequence properties

9 916 497

1 036 381

Filter by sequence properties

12 662 117

3 263 087

t/rRNA removed

9 461 135

8 656 790

1 016 371

841 123

t/rRNA removed

12 195 396

11 213 510

3 246 517

2 773 011

Match known miRNAs

1 132 848

1 117 758

2 423

1 030

Match known miRNAs

664 082

654 984

3 455

1 969

Root 1

Raw reads

4 416 396

Flower 1

Raw reads

17 715 434

Adaptor removed

3 567 326

673 485

Adaptor removed

15 107 462

4 920 011

Filter by sequence properties

3 561 838

669 035

Filter by sequence properties

15 084 084

4 899 263

t/rRNA removed

3 250 883

2 926 826

652 495

553 372

t/rRNA removed

14 374 989

13 270 386

4 872 774

4 186 250

Match known miRNAs

368 908

363 793

1557

827

Match known miRNAs

517 965

510 930

3 461

2 128

Root 2

Raw reads

6 227 488

Flower 2

Raw reads

8 784 135

Adaptor removed

5 472 237

2 020 365

Adaptor removed

8 156 033

2 71814

Filter by sequence properties

5 463 791

2 013 081

Filter by sequence properties

8 143 554

262 570

t/rRNA removed

5 041 719

4 673 608

1 992 006

1 746 022

t/rRNA removed

7 295 596

6 508 891

253 399

185 299

Match known miRNAs

308 179

304 276

2 402

1 474

Match known miRNAs

817 620

807 317

1 526

547

Leaf 1

Raw reads

4 481 051

Leaf ^BTI

Raw reads

2 646 118

Adaptor removed

3 752 733

1 675 147

Adaptor removed

2 373 816

445 460

Filter by sequence properties

3 746 943

1 670 236

Filter by sequence properties

2 370 062

442 372

t/rRNA removed

3 567 987

3 252 343

1 644 909

1 440 195

t/rRNA removed

2 277 189

2 092 901

433 297

375 749

Match known miRNAs

258 610

255 179

2 368

1 502

Match known miRNAs

234 022

231 082

1 025

489

Leaf 2

Raw reads

3 801 050

Seedling ^BTI

Raw reads

7 874 296

Adaptor removed

2 925 945

1 149 340

Adaptor removed

7 174 326

2 105 046

Filter by sequence properties

2 921 408

1 145 456

Filter by sequence properties

7 163 048

2 095 876

t/rRNA removed

2 834 354

2 596 026

1 131 710

982 432

t/rRNA removed

6 906 776

6 088 762

2 070 845

287 724

Match known miRNAs

274 028

270 476

1 434

737

Match known miRNAs

786 266

423 581

2205

113