Fig. 3
From: Genome editing through large insertion leads to the skipping of targeted exon
RT-PCR analysis to confirm expression of wild type and exon-skipped transcripts. Gel images for RT-PCR products of hCDC14A (a) and hCDC14B (b) transcripts from wild type (Wt) and knockout cells (KO). Maps show the locations of primers (black arrows) used for PCR reaction. The generated DNA bands were gel purified and sequenced. Sequences of alternating exon junctions are shown in the respective lower panels (exon 8 to 10 in case of hCDC14A and exon 3 to 5 for hCDC14B). In-frame exon skipping can be deduced from the amino acid sequences written above the codons