Fig. 5

Biosynthetic gene cluster and antibacterial activity associated with tripropeptin A production by the Collimonas stains. a Organization of the gene cluster and predicted amino acid composition of the Tripropeptin A in C. fungivorans Ter331 and Ter6 genomes. Underneath the genes are the module and domain organization of trpA, trpB and trpC. The domains are as follows: C, condensation; A, adenylation; T, thiolation; and TE, thioesterification. Underneath the domains are the amino acids that are incorporated into the LP peptide moiety. The number associated with the amino acid refers to the position of the amino acid in the LP peptide chain. The black triangle indicates the position of the Gm cassette insertion in the trpA gene. b Antibacterial activity associated with tripropeptin A production. Strains Ter331, Ter6, Δ13E12 mutant (deficient in collimomycin biosynthesis), ΔNLP mutant (deficient in the new lipopeptide biosynthesis) and ΔNLP13E12 mutant (deficient in both-new lipopeptide and collimomycin biosynthesis) exhibited inhibition against Staphylococcus aureus via overlay assay. ΔtrpA mutant of C. fungivorans Ter331 (deficient in tripropeptin A biosynthesis)- loss in the inhibition activity. Biosynthetic gene cluster and antifungal/oomycetal activities associated with new lipopeptide production by the Collimonas stains. c Organization of the gene cluster and predicted amino acid composition of the new lipopeptide in C. fungivorans Ter331, Ter6 and C. pratensis Ter91, Ter291 genomes. Underneath the genes are the module and domain organization of NLP. The domains are as follows: C, condensation; A, adenylation; T, thiolation; and TE, thioesterification. Underneath the domains are the amino acids that are incorporated into the LP peptide moiety. The number associated with the amino acid refers to the position of the amino acid in the LP peptide chain. The black triangle indicates the position of the Gm cassette insertion in the nlp gene. d Antifungal/oomycetal activities associated with new lipopeptide production. Strain Ter331 which has new lipopeptide biosynthetic clusters exhibited inhibition against Fusarium culmorum, Rhizoctonia solani and Saprolegnia parasitica. Mutant ΔNLP of Ter331 deficient in new lipopeptide biosynthesis abolished the inhibition activities. CK is control grew without bacteria