Fig. 6

a Antimicrobial activities of pure β-pinene and 4-VOCs (mix of β-pinene, α-pinene, myrcene and terpinene in 1:1:1:1 ratio) against Staphylococcus aureus, Escherichia coli and Rhizoctonia solani. CK is control without terpenes. 5 mm sterilized white filter papers were placed in the centre or on the bottom of the petri dishes for antibacterial and antifungal assays respectively. 2 μl of each VOC was added accordingly to the white filter papers. b Phylogenetic tree of characterized bacterial terpene cyclase proteins. The protein name indicates the bacterial species and the major terpene produced by these terpene cyclases. Sequences included are listed in Additional file 2: Table S13. c Ter91 terpene synthase with FPP. TIC 100 % = 1.76E5. Major sesquiterpene product at RT 15.17, identified as Germacrene D-4-ol by comparison of mass spectra to NIST14 spectral library