Structure, evolution and functional inference on the Mildew Locus O (MLO) gene family in three cultivated Cucurbitaceae spp.

Table 4 Average nucleotide diversity (π) and non synonymous to synonymous substitutions mean dissimilarity analysis

Aligned coding sequences	MLO coding sequences (No)	π	δ	ω
C. pepo MLO family	11	0.21	−17.54	0.41
C. lanatus MLO family	14	0.33	−23.89	0.31
C. melo MLO family	12	0.34	−22.92	0.29
Cucurbitaceae MLO clade I	9	0.31	−18.42	0.31
Cucurbitaceae MLO clade II	4	0.09	−10.47	0.34
Cucurbitaceae MLO clade III	9	0.19	−23.65	0.32
Cucurbitaceae MLO clade V	12	0.22	−27.73	0.27
Cucurbitaceae MLO clade VI	2	0.32	−6.53	-

All the values of δ (=d_N-d_S) and ω (=d_N/d_S) reported in the table, estimated from different alignments of MLO coding sequences, were calculated using the Nei-Gojobori and SLAC methods, respectively, and are significant (p < 0.05 and p < 0.1) over the null hypothesis of strict-neutrality (d_N = d_S;d_N/d_S = 1) in favour of the alternative hypotheses of positive (d_N > d_S;d_N/d_S > 1) or negative (d_N < d_S; d_N/d_S < 1) selections

ISSN: 1471-2164