Fig. 5

Validation of gene expression in dark and white skin by quantitative PCR. Some identified melanogenic genes and lncRNAs were examined in dark and white skin of fetal goats using quantitative PCR. Gene expression was quantified relative to the expression level of β-actin using the comparative cycle threshold (∆CT) method. Correction for multiple comparisons was performed using the Holm-Sidak method. The data are expressed as the mean ± 1 SE (n = 3). * P < 0.05, **P < 0.01, *** P < 0.001