Fig. 3

Preparation of Southern bluefin tuna gonad samples for RNA extraction. a – Gonad dissection (ovaries from females and testes from males); b – Enzymatic digestion of the crude gonads to break down the tissue to individual cells; c – Percoll gradient enrichment of ASG from the testes cells; d – Filtration through a 30 μm pore size filter to enrich oogonia cells from the ovary cells; e – RNA extraction from each of the 4 groups (testes/ovaries, both crude/enriched)