Fig. 1

The scheme illustrating the developed workflow of lampbrush chromosomes (LBC) microdissection. The main steps are as follows: 1. Mechanical microdissection of lampbrush chromosome regions; 2. Primary amplification of isolated DNA material by DOP-PCR. Alternatively, for investigation of RNA-component of chromosomal loci, before the amplification the dissected material was pretreated with DNAse I followed by reverse transcription; 3. FISH-probe generation via reamplification and labeling; 4. Verification of specificity and brightness of the probes by FISH on metaphase chromosomes; 5. High-resolution FISH-mapping of dissected regions on lampbrush chromosomes; 6. Preparation of DNA-libraries for high-throughput sequencing. Processing, visualization and analysis of the sequencing data using web-based bioinformatic platform Galaxy [39] and genome browser IGV [43, 44]. The sequence of actions is depicted by arrows