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Fig. 2 | BMC Genomics

Fig. 2

From: Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish

Fig. 2

CRISPR workflow. a Overall workflow. Diagram showing the steps of the process. b Strategy for sgRNA screening. Initially, sgRNAs are screened for efficiency and those with high cutting efficiency are re-injected. The G0 embryos are raised and males are screened for germline CRISPR-induced indels. For high-transmitting samples, embryos are generated by IVF, raised and the resulting F1 carriers are identified by KASP

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BMC Genomics

ISSN: 1471-2164

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