Fig. 3

Analysis of the HCV quasispecies utilising near full-length reads with the PacBio RS platform. Individual sequences reads greater than 9 kb were generated and aligned for an HCV infected individual, RIMM. Panel (a). Highlighter plot (www.hiv.lanl.gov/content/sequence/HIGHLIGHT/highlighter.html) representing 45 unique haplotypes that were detected from sequence reads. The name of each haplotype shows the frequency of occurrence within the population (e.g. HAP_0.11). The master sequence on the top of the plot is the consensus genome sequence obtained from the Illumina sequences. Panel (b). Phylogenetic tree showing the genetic relationship between near-full length quasispecies obtained from PacBio reads