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Table 4 Time-course analysis of gene expression of orsellinic acid and monodictyphenone synthases and major regulators

From: Transcriptomic and metabolomic profiling of ionic liquid stimuli unveils enhanced secondary metabolism in Aspergillus nidulans

   

qRT-PCRa

Media

GeneID

Gene

5d

10d

15d

Choline

AN7909

orsA

2.04

−3.62

1.49

 

AN0150

mdpG

−57.33

−44.94

−2.66

 

AN0807

laeA

−10.09

−4.32

−10.00

 

AN4562

rsmA

−1.64

1.43

2.30

 

AN1052

veA

−2.91

−1.77

−1.00

[C2mim]Cl

AN7909

orsA

24.65

1.29

3.42

 

AN0150

mdpG

−5.54

−74.07

−39.50

 

AN0807

laeA

−6.93

−13.87

−31.40

 

AN4562

rsmA

1.94

−2.02

1.09

 

AN1052

veA

−4.14

−6.85

1.65

  1. qRT-PCR analysis of the expression of orsA and mdpG (backbone genes of orsellinic acid and monodictyphenone, respectively), and laeA, veA and rsmA (secondary metabolism regulatory genes) along the incubation time in choline or 1-ethyl-3-methylimidazolium chloride ([C2mim]Cl) supplemented media. Values represent relative gene expression at each culture time in pair-wise comparisons with the control. Expression of each gene was normalised to the expression of the histone protein H3 gene (AN0733)
  2. aFold changes (FCs) in pair-wise comparison with the control
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BMC Genomics

ISSN: 1471-2164

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