Fig. 2

Micromonas pusilla and M. commoda cellular structures and gene model sets. Transmission electron micrographs of CCMP1545 (a) and RCC299 (b) show the chloroplast (Cp) comprising more than half the cell, the pyrenoid (Py), nucleus (Nu) and flagellum (Fl) with microtubules visible (24 nm diameter). In RCC299 the beginning of the flagellar hair (Fh) is also visible as are the thylakoid membranes in CCMP1545 (Th). Evidence for new gene models in CCMP1545 (c) and RCC299 (d) is shown based on: Function: Predicted protein had a functional domain hit in Interproscan; Transcribed: Predicted gene model was expressed (FPKM > 2,000) in the RNA-Seq experiment; LC-MS Peptides: At least one peptide matching the predicted protein was identified in the LC-MS/MS analysis; Conserved: Blastp hits were found in NCBI’s nr database (E-value ≤10⁻¹⁵). The total number of genes with no evidence: 25 (CCMP1545) and 37 (RCC299). Note that for RCC299 the lower number of gene models supported by LC-MS/MS data (2,306) than in CCMP1545 (8,432) is due to generation of fewer peptides