Fig. 1

Schematic representation of the bprR (bpss0688) and bprS (bpss0687) genes in the B. pseudomallei K96243 wild-type strain and each of the bprS and bprR mutants. Panel a, organisation of the bprRS genes in B. pseudomallei wild-type showing the conserved amino acid domains encoded within each gene. Domains shown are the receiver and effector domains within bprR and, within bprS, the following domains: transmembrane regions 1 and 2 (TMR1 and 2) between which the stimulus-specific region is located, the HAMP (histidine kinases, adenylyl cyclases, methyl-accepting chemotaxis proteins, and phosphatases) signal transduction domain, the HisKA (histidine kinase A) dimerization/phosphoacceptor domain and the HATPase_c (histidine kinase-like ATPase catalytic) domain. The relative position of the insertion in each of the single mutants is shown below the gene schematic. Panel b, schematic representation of the regions remaining in each gene and the relative position of the tetracycline gene cassette in the bprRS double mutant