Perturbation of the two-component signal transduction system, BprRS, results in attenuated virulence and motility defects in Burkholderia pseudomallei

Table 4 Regulatory genes differentially expressed in the bprR and/or bprS single mutants

Locus tag/gene	Description/predicted function	Expression in bprS mutant	Expression in bprR mutant
Two component signal transduction systems				Class/Function
BPSL1829	methyl-accepting chemotaxis protein	Increased	Increased#	MCP/Chemotaxis
BPSS0063 dctD	C4-dicarboxylate transport protein	Decreased	NS	RR/Receiver
BPSS0215 tar	methyl-accepting chemotaxis protein	Increased	Increased#	MCP/Chemotaxis
BPSS0687 bprS	sensor kinase protein	Increased	Increased	HK/Transmitter
BPSS0688 bprR	response regulator protein	Increased	Increased	RR/Receiver
One component regulators				Domain-input	Domain-output
BPSL0812 bpeR	TetR family regulatory protein	Increased	Increased	Not known	TetR_N-DNA binding
BPSL1564	transcriptional regulatory protein	NS	Increased	Not known	HTH_26-DNA binding
BPSL2972	IclR family regulatory protein	Decreased	NS	IclR/Small-molecule binding	HTH_IclR-DNA binding
BPSL3291 fliA	flagellar biosynthesis sigma factor	Increased	Increased	Not known	Sigma70 -DNA binding
BPSS0070	IS30 transposase	NS	Decreased	Not known	HTH_38-DNA binding
BPSS0312 malR	LuxR transcriptional regulator	Increased	Increased	Autoind_bind- Small-molecule binding	GerE-DNA binding
BPSS0755	LysR family regulatory protein	NS	Increased	LysR_substrate- Small-molecule binding	HTH_1-DNA binding
BPSS0797	IclR family regulatory protein	NS	Increased	IclR/Small-molecule binding	HTH_IclR-DNA binding
BPSS0799	Diguanylate phosphodiesterase	Increased	Increased		EAL/Di-guanylate cyclase
BPSS1553 bprP	Transcriptional regulator	Increased	Increased		Trans_reg_C/DNA-binding

Genes were identified as differentially expressed if they showed >2-fold expression change (log₂ < −1.0) compared to the wild-type strain with a False Discovery Rate (FDR) of <0.01
^#Genes showing increased expression of >2-fold with a FDR of >0.01 and <0.05

ISSN: 1471-2164