Fig. 4
From: High-specificity detection of rare alleles with Paired-End Low Error Sequencing (PELE-Seq)
PELE-Seq data has zero false positive SNPs and high sensitivity. Sequencing a control E. coli DNA library containing 64 expected rare SNPs present at 0.42 % average allele frequency with PELE-Seq at 20,000× OPE read depth (48,000× raw read depth) produces 100 % specific data, compared to 75 % specificity achieved with standard sequencing methods. Standard DNA-Seq of the control libraries resulted in 12 false positive mutations, compared to zero with the PELE-Seq method