Fig. 5

Rare SNPs present at 0.42 % frequency, detected with PELE-Seq and standard DNA-Seq methods. A control E. coli library containing rare alleles present at 0.42 % frequency were sequenced with PELE-Seq and standard DNA-Seq with 20,000× OPE depth (48,000 non-overlapped read depth). The read depths of the individual barcode files are plotted in light green, and the total read depth is plotted in blue. The SNPs detected with PELE-Seq are plotted in the inner circle, and the standard DNA-Seq SNPs are plotted in the next outer circle. The 12 false positive SNP calls present in the standard DNA-Seq data are designated with a red “X”. Of the 64 expected rare SNPs, PELE-Seq detected 42 SNPs with 100 % specificity, compared to 36 SNPs with 75 % specificity achieved with standard DNA-Seq methods