High-specificity detection of rare alleles with Paired-End Low Error Sequencing (PELE-Seq)

BMC Genomics

Table 3 Rare SNPs in the wild C. remanei population that significantly increase in frequency after lab-adaptation

Position	Ref	Alt	Wild allele frequency	Wild allele depth	Lab allele frequency	Lab allele depth
2933656	G	A	0.010233	11	0.111517	61
84255709	C	A	0.018789	27	0.17717	149
89350272	A	G	0.018276	60	0.122693	379
114867644	G	A	0.017125	43	0.164325	386
127723967	C	G	0.00235	31	0.103739	738
138506868	A	C	0.013662	31	0.163548	236
141293514	T	C	0.018643	25	0.275908	205

Seven SNPs found below 2 % frequency in the wild C. remanei population increased in frequency at least five-fold in the lab-adapted population. The read depths listed are those of the detected rare allele, not the total read depth at that position. Read depths listed are for overlapping paired-end (OPE) reads

ISSN: 1471-2164