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Table 4 Validation of PR gene induction by qRT-PCR

From: Theobroma cacao L. pathogenesis-related gene tandem array members show diverse expression dynamics in response to pathogen colonization

  

P. palmivora treatment

C. theobromicola treatment

 

Gene ID

Microarray fold induction

qRT-PCR fold induction

Microarray fold induction

qRT-PCR fold induction

PR-1 s

Tc01_g003940

N.S.

Transcript not detected

N.S.

Transcript not detected

Tc01_g034430

N.S.

N.S.

N.S.

N.S.

Tc02_g002380

N.S.

N.S.

N.S.

N.S.

Tc02_g002390

N.S.

N.S.

N.S.

N.S.

Tc02_g002400

N.S.

N.S.

N.S.

8.3 (p = .001)

Tc02_g002410

125.4

763 (p < .001)

91.3

55.7 (p < .001)

Tc02_g002420

N.S.

N.S.

N.S.

N.S.

Tc02_g002430

N.S.

N.S.

N.S.

N.S.

Tc02_g010380

N.S.

N.S.

N.S.

N.S.

Tc05_g005530

N.S.

N.S.

N.S.

N.S.

Tc09_g000720

N.S.

N.S.

N.S.

N.S.

Tc09_g016580

N.S.

N.S.

N.S.

N.S.

Tc09_g016590

N.S.

N.S.

N.S.

N.S.

Tc10_g000980

N.S.

Transcript not detected

N.S.

Transcript not detected

PR-3 s

Tc01_g000770

33.6

70.2 (p < .001)

18.8

13.8 (p = .01)

Tc02_g003890

27.1

Transcript not detected

22.31

Transcript not detected

Tc04_g018100

22.5

5086.0 (p < .001)

8.3

36.7 (p = .019)

Tc04_g018110

29.2

763.2 (p < .000)

11.5

13.7 (p = .041)

Tc04_g018160

63.6

158.4 (p = .003)

73

65.6 (p = .001)

Tc06_g000490

N.S.

3.4 (p = .016)

N.S

N.S.

PR-4 s

Tc00_g012980

N.S.

N.S.

N.S.

N.S.

Tc05_g027210

24.9

1027.7 (p < .001)

14.9

22.7 (p = .01)

Tc05_g027220

11.1

258.9 (p = .001)

6.7

N.S.

Tc05_g027230

N.S.

164.1 (p = .011)

N.S.

N.S.

Tc05_g027250

N.S.

N.S.

N.S.

N.S.

Tc05_g027320

53.4

29.3 (p = .009)

17.8

8.9 (p = .001)

Tc10_g011130

N.S.

61.5 (p < .001)

N.S.

N.S.

PR-10s

Tc01_g031100

39.7

28.0 (p = .019)

57.2

32.3 (p = .002)

Tc04_g028780

25.5

32.9 (p = .027)

25.5

41.6 (p = .004)

Tc04_g028860

6.02

24.3 (p = .038)

53.4

96.8 (p = .001)

  1. Genes shown as induced by microarray had BH p-values < 0.05. N.S. indicates p-values for fold change were > 0.05. Inductions detected by qRT-PCR were calculated using REST software [96] and represent the average of five pathogen-treated samples compared to five water-treated samples relative to TcTub1 (Tc06_g000360). Transcripts were considered undetected if the average Ct value across all treatments was greater than 35