Fig. 1
From: RNA-Seq following PCR-based sorting reveals rare cell transcriptional signatures
Workflow for droplet-based rare cell enrichment and analysis. a Single cells from a heterogeneous population are encapsulated in microfluidic droplets and lysed (b). The cell content is merged with RT-PCR reagents, and single cell TaqMan assays are performed in droplets. PCR-Activated Cell Sorting (PACS) allows the recovery of droplets with the desired TaqMan fluorescence profile (c). After breaking the emulsion, the nucleic acids are recovered and used for downstream analysis, including library preparation for RNA-Seq (d) and gene expression analysis (e)