Fig. 8

Time-course Northern blotting analysis of SR73. RNA was isolated from the wild-type JIR325 cultured in TPG broth at the indicated times (1, 2, 3, 4 and 5 h). A total of 10 μg of RNA extracted from the wild-type strain was used in Northern blotting analysis to examine the expression of SR73. The loading control shows the total RNA samples that were separated by agarose gel electrophoresis and later transferred to a Nylon membrane for Northern blot analysis. The genes flanking SR73 are indicated as green arrows, while the yellow bar shows the position of the SR73 probe. The probe was generated using forward (→) and reverse (←) primers as designated in the gene map. The hybridization of the probe to the target RNA was detected by CDP-Star chemiluminescence. The black solid arrow marks the transcript that hybridized to the probe and the estimated size of the transcripts (in kb) is shown on the right of the Northern blot image