Table 1 Selected deletion strains sensitive to both TTC and DON

5

YMR078C

CTF18

3

3

DNA damage

Subunit of a complex with Ctf8p

6

YOR299W

BUD7

3

4

Golgi

Member of the ChAPs family

7

YEL036C

ANP1

3

2

Golgi

Subunit of the alpha-1,6 mannosyltransferase complex

2

8

YPR051W

MAK3

3

2

Golgi

Catalytic subunit N-terminal acetyltransferase

9

YOR147W

MDM32

4

4

Mitochondria

Mitochondrial inner membrane protein

10

YOR178C

GAC1

4

4

PP1

Regulatory subunit for Glc7p type-1 protein Pase (PP1)

11

YCL045C

EMC1

2

4

Folding

Member of conserved ER membrane complex

12

YIL084C

SDS3

3

4

Rpd3 HDAC

Component of the Rpd3L histone deacetylase complex

13

YOL004W

SIN3

3

4

Rpd3 HDAC

Component of both the Rpd3S and Rpd3L HDAC

5

14

YPL139C

UME1

3

3

Rpd3 HDAC

Component of both the Rpd3S and Rpd3L HDAC

16

YOR043W

WHI2

3

3

Stress

Required for activation of the general stress response

17

YGL025C

PGD1

3

3

Transcription

Subunit of the RNA polymerase II mediator complex

1

18

YOR039W

CKB2

3

2

Transcription

Beta' regulatory subunit of casein kinase 2 (CK2)

19

YEL007W

MIT1

3

2

Transcription

Transcriptional regulator of pseudohyphal growth

20

YOR298C-A

MBF1

2

4

Transcription

Transcriptional coactivator

3

21

YJL115W

ASF1

2

3

Transcription

Nucleosome assembly factor

4

22

YJL176C

SWI3

2

3

Transcription

Subunit of the SWI/SNF chromatin remodeling complex

1

23

YER064C

VHR2

2

3

Transcription

Non-essential nuclear protein

24

YLR418C

CDC73

2

1

Transcription

Component of the Paf1p complex

25

YMR116C

ASC1

4

4

Translation

G-protein beta subunit

3

26

YDR266C

HEL2

4

4

Translation

RING finger ubiquitin ligase (E3)

29

YGR271W

SLH1

3

4

Translation

Putative RNA helicase related to Ski2p

30

YML034W

SRC1

3

4

Translation

Inner nuclear membrane protein

31

YIR001C

SGN1

2

4

Translation

Cytoplasmic RNA-binding protein

32

YDR049W

VMS1

2

4

Translation

Component of the CCR4-NOT transcriptional complex

3

33

YML111W

BUL2

4

3

Ubiquitin

Component of the Rsp5p E3-ubiquitin ligase complex

34

YEL013W

VAC8

4

3

Vacuole

Vacuolar membrane protein

5

35

YCL048W

SPS22

3

2

Various

Protein of unknown function

1

YDR388W

RVS167

4

4

Cytoskeleton

Actin-associated protein

7

2

YDR129C

SAC6

4

4

Cytoskeleton

Fimbrin, actin-bundling protein

10

3

YLR337C

VRP1

4

4

Cytoskeleton

Verprolin, proline-rich actin-associated protein

7

4

YBL007C

SLA1

2

4

Cytoskeleton

Cytoskeletal protein binding protein

7

15

YLR056W

ERG3

3

4

Stress

C-5 sterol desaturase

12

27

YAL021C

CCR4

4

4

Translation

Component of the CCR4-NOT complex

12

28

YNR052C

POP2

4

4

Translation

Component of the CCR4-NOT complex

10