Table 1 List of the library preparation kits, DNA inputs and adapters tested
Kit | Manufacturer | Reference | DNA inputs (ng) | Adaptors |
|---|---|---|---|---|
NEBNext® | New England Biolabs®Inc. | Cat. #E6040S/L | 500 | Sanger ([1], current protocols) |
NEBNext® Ultra™ | New England Biolabs®Inc. | Cat. #E7370S/L | 500 | Sanger |
SureSelectXT | Agilent | Cat. #930075 | 500 | Sanger |
Truseq® Nano | Illumina® | Cat. # FC-121-9010DOC, Part # 15041110 Rev. B | 500 & 100 | Sanger & Illumina |
Truseq® DNA PCR-free | Illumina® | Cat. # FC-121-9006DOC, Part # 15036187 Rev. B | 500 | Sanger & Illumina |
Accel-NGSâ„¢ 1S | Swift Biosciencesâ„¢ | Cat. No. DL-ILM1S-12/48, Version 04291444 | 500 & 100 | Swift Biosciences |
Accel-NGSâ„¢ 2S | Swift Biosciencesâ„¢ | Cat. No. DL-ILM2-48, Version 01131444/2.8 | 500 & 20 | Swift Biosciences |
KAPA Hyper | KAPA Biosystems | Cat. #KR0961 – v1.14 | 500 | Sanger |
KAPA HyperPlus | KAPA Biosystems | Cat. #KR1145 – v14.1 | 500 & 20 | Sanger |