Fig. 4

Features of the L1 antisense promoter revealed from ESTs, ENCODE ChIP-seq, and GRO-seq data. a ESTs supporting alternative L1 ASP transcripts for L1HS, L1PA2-8, ancient primate L1, and ancient mammalian L1 subfamilies were aligned to full-length L1 consensus sequences. The plot revealed evolutionarily recent L1HS and L1PA2-8 subfamilies possess the ASP activity in the 5’ UTR, whereas ancient primate and ancient mammalian L1 subfamilies display minor ASP activity in the 5’ UTR and the majority is at the 3’ end of the element overlapping the end of ORF2. b YY1 enrichment profile in the 5’UTR of L1HS consensus sequence for ENCODE YY1 ChIP-seq in various cell-lines. The schematic above is a reference point to the L1HS consensus position. c The TSS enrichment profile for YY1, H3K9me2, and H3K9me3 ChIP-seq for L1HS and L1PA2-8 AS ESTs in units of input subtracted reads per million mapping reads (RPM) using the K562 cell-line. The bottom panel displays the GRO-seq TSS enrichment profile in RPM enrichment units. The schematic above is a reference point to the L1HS consensus position